Respiratory Syncytial Virus Infection Induces Expression of the Anti-Apoptosis Gene IEX-1L in Human Respiratory Epithelial Cells
Identifieur interne : 000F42 ( Istex/Checkpoint ); précédent : 000F41; suivant : 000F43Respiratory Syncytial Virus Infection Induces Expression of the Anti-Apoptosis Gene IEX-1L in Human Respiratory Epithelial Cells
Auteurs : Joseph B. Domachowske [États-Unis] ; Cynthia A. Bonville [États-Unis] ; Anthony J. Mortelliti [États-Unis] ; Carol B. Colella [États-Unis] ; Urian Kim [États-Unis] ; Helene F. Rosenberg [États-Unis]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2000.
Abstract
By means of differential display reverse-transcriptase polymerase chain reaction, increased expression of the mRNA encoding the anti-apoptosis gene IEX-1L was found in respiratory epithelial cells infected with respiratory syncytial virus (RSV). IEX-1L mRNA expression increased 5–7-fold in RSV-infected cells at 72 h after infection but remained unchanged in cells exposed to irradiated, replication-incompetent RSV. Because IEX-1L is reported to protect cells from apoptosis induced by tumor necrosis factor (TNF)-α, the effect of TNF-α on epithelial cell apoptosis in the context of RSV infection was determined. Epithelial cells were exposed to vehicle, RSV, or irradiated RSV for 72 h, and then TNF-α was added to appropriate cultures. Cytochemical staining of cellular DNA with 4,6-diamidino-2-phenylindole demonstrated TNF-α-induced apoptosis in 23.4% of control cells but only 5% of RSV-infected cells. These data show that RSV infection protects epithelial cells from TNF-α-induced apoptosis and that this effect is temporally associated with IEX-1L gene expression.
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DOI: 10.1086/315319
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Colella</name></author><author><name sortKey="Kim, Urian" sort="Kim, Urian" uniqKey="Kim U" first="Urian" last="Kim">Urian Kim</name></author><author><name sortKey="Rosenberg, Helene F" sort="Rosenberg, Helene F" uniqKey="Rosenberg H" first="Helene F." last="Rosenberg">Helene F. 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Domachowske</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Pediatrics, Upstate Medical University, Syracuse</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Reprints or correspondence: Dr. Joseph B. Domachowske, Dept. of Pediatrics, Division of Infectious Disease, Upstate Medical University, 750 E. Adams St., Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Bonville, Cynthia A" sort="Bonville, Cynthia A" uniqKey="Bonville C" first="Cynthia A." last="Bonville">Cynthia A. Bonville</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Pediatrics, Upstate Medical University, Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Mortelliti, Anthony J" sort="Mortelliti, Anthony J" uniqKey="Mortelliti A" first="Anthony J." last="Mortelliti">Anthony J. Mortelliti</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Otolaryngology and Communication Sciences, Upstate Medical University, Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Colella, Carol B" sort="Colella, Carol B" uniqKey="Colella C" first="Carol B." last="Colella">Carol B. Colella</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Pediatrics, Upstate Medical University, Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Kim, Urian" sort="Kim, Urian" uniqKey="Kim U" first="Urian" last="Kim">Urian Kim</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Pediatrics, Upstate Medical University, Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Rosenberg, Helene F" sort="Rosenberg, Helene F" uniqKey="Rosenberg H" first="Helene F." last="Rosenberg">Helene F. Rosenberg</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>University Chicago Press</publisher><date type="published">2000</date><biblScope unit="vol">181</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="824">824</biblScope><biblScope unit="page" to="830">830</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">By means of differential display reverse-transcriptase polymerase chain reaction, increased expression of the mRNA encoding the anti-apoptosis gene IEX-1L was found in respiratory epithelial cells infected with respiratory syncytial virus (RSV). IEX-1L mRNA expression increased 5–7-fold in RSV-infected cells at 72 h after infection but remained unchanged in cells exposed to irradiated, replication-incompetent RSV. Because IEX-1L is reported to protect cells from apoptosis induced by tumor necrosis factor (TNF)-α, the effect of TNF-α on epithelial cell apoptosis in the context of RSV infection was determined. Epithelial cells were exposed to vehicle, RSV, or irradiated RSV for 72 h, and then TNF-α was added to appropriate cultures. Cytochemical staining of cellular DNA with 4,6-diamidino-2-phenylindole demonstrated TNF-α-induced apoptosis in 23.4% of control cells but only 5% of RSV-infected cells. These data show that RSV infection protects epithelial cells from TNF-α-induced apoptosis and that this effect is temporally associated with IEX-1L gene expression.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Domachowske, Joseph B" sort="Domachowske, Joseph B" uniqKey="Domachowske J" first="Joseph B." last="Domachowske">Joseph B. Domachowske</name></region><name sortKey="Bonville, Cynthia A" sort="Bonville, Cynthia A" uniqKey="Bonville C" first="Cynthia A." last="Bonville">Cynthia A. Bonville</name><name sortKey="Colella, Carol B" sort="Colella, Carol B" uniqKey="Colella C" first="Carol B." last="Colella">Carol B. Colella</name><name sortKey="Domachowske, Joseph B" sort="Domachowske, Joseph B" uniqKey="Domachowske J" first="Joseph B." last="Domachowske">Joseph B. 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